American Society of Hematology

Figure 1.

Viral load modulates NK cell activation and phenotype after acute MCMV infection. (A) Viral loads in livers of C57BL/6 mice at day 6 after LD (2 × 103 PFU) or HD (2 × 104 PFU) MCMV infection determined by quantitative PCR of the IE1 gene copy number per 100 ng of DNA. (B-D) Serum proinflammatory cytokines on day 6 after LD and HD MCMV infection. (E) Representative dot plots of Thy1.2 expression on NK cells on postinfection day 6, quantified in the spleen (F) and liver (G). (H) Representative dot plots of KLRG1 expression on NK cells on postinfection day 6, quantified in the spleen (I) and liver (J). (K) Representative staining of Ly49H+ NK cells in the spleen (L) and liver (M) on post-MCMV infection day 6. Means ± standard error of the mean are shown for 3 or 4 mice per group, representative of 2 experiments. *P < .05; **P < .01; ***P < .001; ****P < .0001, by Student t test or 1-way ANOVA with Tukey post hoc test.

Viral load modulates NK cell activation and phenotype after acute MCMV infection. (A) Viral loads in livers of C57BL/6 mice at day 6 after LD (2 × 10³ PFU) or HD (2 × 10⁴ PFU) MCMV infection determined by quantitative PCR of the IE1 gene copy number per 100 ng of DNA. (B-D) Serum proinflammatory cytokines on day 6 after LD and HD MCMV infection. (E) Representative dot plots of Thy1.2 expression on NK cells on postinfection day 6, quantified in the spleen (F) and liver (G). (H) Representative dot plots of KLRG1 expression on NK cells on postinfection day 6, quantified in the spleen (I) and liver (J). (K) Representative staining of Ly49H⁺ NK cells in the spleen (L) and liver (M) on post-MCMV infection day 6. Means ± standard error of the mean are shown for 3 or 4 mice per group, representative of 2 experiments. *P < .05; **P < .01; ***P < .001; ****P < .0001, by Student t test or 1-way ANOVA with Tukey post hoc test.

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