Figure 5.
ChIP-seq analyses in the presence of EZH2 inhibition during MK differentiation. (A) Peak distribution along the gene regions as indicated on the right side. (B) ChIP-seq profiles of the 3 histone marks (H3K4me3, H3K27me3, and H3K27ac) across CDKN1A (p21) (blue, control; red, GSK343 treated at day 12 of culture) are shown in the upper panel and ChIP-EZH2 qPCR in the lower panel. (C) ChIP-seq profiles of the 3 histone marks (H3K4me3, H3K27me3, and H3K27ac) across CDKN2D (p19) (blue, control; red, treated with GSK343 at day 12 of culture) are shown in the upper panel and ChIP-EZH2 qPCR in the lower panel. (B-C) In the lower panel, 1 representative ChIP experiment of 3 experiments is shown in duplicate on the left, and the statistical analysis of 3 independent ChIP experiments is shown on the right. Data were compared using a Student t test with Mann-Whitney correction. All data represent means ± SEM. *P < .05.

ChIP-seq analyses in the presence of EZH2 inhibition during MK differentiation. (A) Peak distribution along the gene regions as indicated on the right side. (B) ChIP-seq profiles of the 3 histone marks (H3K4me3, H3K27me3, and H3K27ac) across CDKN1A (p21) (blue, control; red, GSK343 treated at day 12 of culture) are shown in the upper panel and ChIP-EZH2 qPCR in the lower panel. (C) ChIP-seq profiles of the 3 histone marks (H3K4me3, H3K27me3, and H3K27ac) across CDKN2D (p19) (blue, control; red, treated with GSK343 at day 12 of culture) are shown in the upper panel and ChIP-EZH2 qPCR in the lower panel. (B-C) In the lower panel, 1 representative ChIP experiment of 3 experiments is shown in duplicate on the left, and the statistical analysis of 3 independent ChIP experiments is shown on the right. Data were compared using a Student t test with Mann-Whitney correction. All data represent means ± SEM. *P < .05.

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