Frequency and location of AGSs in the Ig heavy chain variable region of DLBCL subsets. Tumor IGHV-IGHD-IGHJ-IGHC rearrangements from primary DLBCL cases were analyzed according to the IMGT/V-QUEST numbering system, and NxS/T motifs acquired by somatic hypermutation (AGSs) were identified. The pie charts show the frequency of rearrangements with at least 1 AGS or no AGSs. Bar charts identify the percentage of rearrangements with sites containing at least 1 AGS in the CDR or AGSs in the FR only . (A) Homology (%) of the tumor IGHV transcript sequence to the closest germline sequence in GCB-, ABC- and unclassified DLBCLs. Horizontal bars indicate the mean. (B) Frequency of rearrangements with AGSs (pie charts) and their distribution in the CDR or FR only (bar charts), divided by COO subset (GCB-, ABC- and unclassified DLBCLs). (C) Frequency of rearrangements with AGSs in the CDR or the FR only relative to the total number of GCB-, ABC- and unclassified DLBCLs. (D) Frequency of rearrangements with AGSs (pie chart) and their distribution in the CDR or FR only (bar chart) in the ABC-DLBCL using IGHV4-34. (E) Distribution of genetic subtypes (according to LymphGen algorithm) within GCB-DLBCL having acquired sites in the CDR. (F) Frequency of rearrangements with AGSs (pie chart) and their percent distribution in the CDR or FR only (bar chart) in GCB-DLBCLs assigned to the EZB genetic subtype according to the LymphGen algorithm. +ve, positive; ns, not significant.