DC-SIGN binds specifically to the sIg-Mann GCB-DLBCL cells. Binding of recombinant DC-SIGN was analyzed in primary samples and DLBCL cell lines by flow cytometry after incubation with fluorescein isothiocyanate (FITC)-conjugated anti-Fc antibody. Blue lines represent binding of DC-SIGN, red lines represent binding of the secondary antibody in the untreated control. (A) DC-SIGN binding to the clonal CD20⁺/CD10⁺ of GCB-DLBCL primary samples or CD20⁺/CD10^– ABC-DLBCL tumor population primary samples. (B) DC-SIGN binding in GCB-DLBCL (with AGSs in the CDR or in the FR only) and ABC-DLBCL cell lines (not having AGSs). Supplemental Tables 6 and 7 show the intensity of DC-SIGN binding to the primary samples and cell lines. NT, untreated.