Histone 4 acetylation and chromatin condensation. (A) Representative western blots showing changes in histone H4 acetylation (AC-H4) during terminal erythroid differentiation. (B) Representative western blots showing effects of HDAC5 knockdown on H4 acetylation on K5, K8, K12, and K16. (C) Quantitative analysis of H4K12 acetylation levels from 3 independent experiments. (D) Representative ImageStream images of day 17 erythroblasts stained with anti-H4K12, GPA, and Hoechst 33342. (E) Quantitative analysis of H4K12⁺ erythroblasts. (F) Representative nuclear area profiles of H4K12⁺ and H4K12⁻ erythroblasts from ImageStream analysis. (G) Quantitative analysis of nuclear area of H4K12⁺ and H4K12⁻ erythroblasts from 3 different experiments. (H) Effect of LMK235 on H4K12 acetylation in polychromatic and orthochromatic erythroblasts. (I) Effect of LMK235 on chromatin condensation (a) and enucleation (b) of polychromatic erythroblasts. (J) Effect of LMK235 on chromatin condensation (a) and enucleation (b) of orthochromatic erythroblasts. *P < .05; **P < .01; ***P < .001. NS, nonsignificant. Ortho, orthochromatic erythroblasts; Poly, poly chromatic erythroblasts.