CCL5 expression in DLBCL correlates with TAM. (A) Tonsil (upper panels) and DLBCL biopsies (lower panels) were stained for CD68 and CD45. The photographs are representative of ≥10 tonsils and 10 DLBCLs. Scale bars, 10 µm. (B) Lysates of 2 DLBCL biopsies were profiled for chemokine expression (left panels). Duplicate dots corresponding to CCL5 are shown. Dots corresponding to other monocyte-acting chemokines are underlined. A quantification by pixel intensity is provided for the monocyte-specific chemokines (right panel). (C) CCL5 was quantified in 7 additional DLBCL biopsy lysates by ELISA. (D) Correlation plots for CCL5 and CD68 or CD14 mRNA expression in tumor lesions from the GSE10846 DLBCL dataset. P values and Pearson correlation coefficients (r) are shown. (E) Reverse transcription PCR analysis of CCL5 mRNA expression in the CD20⁺ and CD20⁻ fractions obtained from primary DLBCL samples. (F) CCL5 concentration in the supernatant of nonstimulated (NS), CD40L/α-BCR, and TNF-stimulated DLBCL cell lines. The figure is representative of ≥5 experiments. Data are mean ± standard deviation. *P < .05 vs NS group.