Anti-CD36 antibodies impaired vascularization in the immunized Cd36^−/− mice placenta labyrinth zone. Additional 3 naive or Cd36^−/− immunized female mice (n = 3) were crossed with WT male mice. The labyrinth zone was quantitatively analyzed at 16.5 days’ postcoitum. (A) The solid line shows the labyrinth area (scale bars, 500 µm). (B) The labyrinth ratio to the whole placenta is shown as a box-and-whisker plot. (C) Representative photomicrographs of the naive and immunized Cd36^−/− placenta labyrinth areas stained with anti-CD31 antibodies. Scale bars, 50 µm. (D) Numbers of fetal capillaries in the labyrinth areas. Data are expressed as mean ± standard deviation . Significance (**P < .005; ***P < .0005) were analyzed by using a two-tailed unpaired Student t test. JZ, junctional zone; LB, labyrinthine layer; MD, maternal decidua.