Expression of MAPK family members during terminal human erythroblast differentiation. RNA-sequencing data showing expression of ERK (A), p38 (B), and JNK (C) in sorted erythroblasts cultured from cord blood CD34⁺ cells. Bar plot represents mean ± standard deviation of triplicate samples. (D-F) Western blot analyses of ERK1/2 and pERK1/2 in erythroblasts cultured for different days as indicated. (G-I) Western blot analyses of p38 and pp38 in erythroblasts cultured for different days as indicated. (J-L) Western blot analyses of JNK and pJNK in erythroblasts cultured for different days as indicated. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the loading control. N = 3. EB, early basophilic erythroblast; FPKM, fragments per kilobase of transcript per million mapped reads; LB, late basophilic erythroblast; Ortho, orthochromatic erythroblast; Poly, polychromatic erythroblast; Pro, proerythroblast.