ERK inactivation inhibited enucleation. (A) Representative western blot showing changes in phosphorylation of ERK1/2, p38, and JNK in orthochromatic erythroblasts treated with specific inhibitor as indicated. N = 3. (B) Representative flow cytometry profiles showing the effects of various inhibitors on enucleation of orthochromatic erythroblasts. (C) Quantitative analyses of enucleation on orthochromatic erythroblasts. N = 3. Data are expressed as mean ± standard deviation. (D) Representative cytospin images of orthochromatic erythroblasts treated with DMSO or specific inhibitor as indicated. Scale bar, 10 μm. *P < .05, **P < .01, ***P < .001. n.s., not statistically significant.