Acquisition of resistance mutations. The ternary plot shows the clonal evolution from pretreatment to cycle 3, day 1 ctDNA in each gene and among all histologies. Only nonresponding/progressing patients were included in the analysis, corresponding to a total of 406 mutations analyzed (nonsynonymous somatic mutations and noncoding mutations affecting regions of ASHMs). Each circle indicates a gene. The genes of interest are labeled. The size of each circle is proportional to the number of somatic nonsynonymous gene mutations. Color codes and the position of the circles on the side of the ternary plot define whether genes have mutations that are preferentially pretreatment-private (left, blue), progression-private (right, yellow), or shared mutations (top, red).