miR-497/195 overexpression impairs leukemia engraftment. (A) Experimental layout of in vivo experiment with transduced BCP-ALL cells from the PDX-S2 sample. (B-C) Leukemia loads of human CD19 (huCD19) and mOrange2-positive cells isolated in spleen and bone marrow of euthanized mice 10 weeks after transplantation of 105 cells per recipient. Results are pooled from 2 independent experiments. Comparison of empty vector (ev) control and miR-497/195 overexpression (Mann-Whitney U test, horizontal lines). (D) Kaplan-Meier curve for mice transplanted with 2 × 104 mOrange2-positive cells isolated from mice in panel B (log-rank test). (E-F) Experimental layout and Kaplan-Meier curve for mice transplanted with 1.8 × 105 transduced cells of PDX-S7 (log-rank test). (G-H) Experimental layout and Kaplan-Meier curve for mice transplanted with 5 × 105 transduced cells of PDX-L8 (log-rank test). In all experiments the same cell number was transplanted in empty vector (ev) control and miR-497/195 groups. Data are presented as mean ± SD.