Expression of HO-1, IKBα, p21, FPN, and RNR2 in SCT PBMCs. Cell lysates from activated PBMCs obtained from individuals with SCT and normal control subjects were resolved on 4% to 12% Bis-Tris gel and probed with antibodies against HO-1 (A), IKBα (B), p21 (C), FPN (D), and RNR2 (E). β-actin was used as loading control. Results were quantified by using ImageQuant Software (Cytiva, Marlborough, MA). Bars represent 2 independent experiments. The blots show 2 representative SCT and control samples (except RNR2, where 3 SCT samples are shown). Total number of samples analyzed and quantified were 4 HbAA (50% female) and 4 HbAS (75% female) (panels A-D) and 4 HbAA (25% female) and 4 HbAS (100% female) (panel E). Data are shown as mean ± standard deviation. P values were calculated by using the Student t test.