Elevated levels of HO-1, SAMHD1, and RNR2 in PBMCs from SCT individuals with HIV-1. (A-G) RNA was extracted from buffy coats obtained from HIV-1–positive HbAS and HbAA individuals. RNA was reverse transcribed and analyzed by real-time PCR using 18S RNA as a reference for ΔΔCt analysis for HIV-1 env (panel A, 9 HbAS [55.6% female] and 12 HbAA [66.7% female]), HO-1 mRNA (panel B, 8 HbAA [75% female] and 9 HbAS [55.6% female]), SAMHD1 mRNA (panel C, 8 HbAA [75% female] and 6 HbAS [50% female]), RNR2 mRNA (panel D, 8 HbAA [75% female] and 6 HbAS [50% female]), IKBα mRNA (panel E, 8 HbAA [75% female] and 9 HbAS [55.6% female]), FPN mRNA (panel F, 8 HbAA [75% female] and 9 HbAS [55.6% female]), and p21 mRNA (panel G, 8 HbAA [75% female] and 9 HbAS [55.6% female]). Data are shown as mean ± standard deviation. P values were calculated by using the Student t test.