EFL1 is required for ribosomal protein (RP) synthesis. (A) Schematic diagram of the RNA expression analysis from K562 ribosomal fractions. (B) Volcano plot of the genes plotting differential enrichments of wild-type expression vs mutant expression in the 80S fraction compared with the 40S fraction (left). GO analysis of the 248 downregulated genes (right). Red dots depict RP genes (GO:0022625 and GO:0022627; n = 118). (C) Fold changes of 80S-enriched genes normalized by 40S, depicted against transcriptome. Red indicates significantly downregulated RP genes in panel B; blue indicates other RP genes. (D) Fractions of 40S-, 80S-, or polysome-bound RNAs for RP genes (n = 108; left), TP53 target genes (n = 51; middle), and all other genes (n = 11 812; right) are displayed. (E) Consensus sequence profile of downregulated RP gene 5' untranslated regions, deducing a terminal oligopyrimidine (TOP) sequence. *P = 4.7 × 10−14, **P = 1.5 × 10−26 by Wilcoxon signed rank test; all other test P values >0.05. KO, knockout; rel. seq. cons., relative sequence conservation from the MEME run; RNA-seq, RNA sequencing; rRNA, ribosomal RNA; WT, wild type.