Mll-AF4⁺ pMIRH-130b/pMIRH-128a⁺ cells display a gene expression profile similar to MLL-AF4⁺ patient blasts. (A) Mll-AF4 expression in transduced/GFP⁺ E14 FL Mll-AF4⁺ LSK (preleukemia and pretransplantation). (B) Heatmap of gene expression in transduced E14 FL Mll-AF4⁺ LSK. (C) Summary of the gene expression results of transduced E14 FL Mll-AF4⁺ LSK. (D) Mll-AF4 expression in total BM cells of control and leukemic mice. (E) Heatmap of gene expression in total BM cells. (F) Summary of the gene-expression results of total BM cells. Data were compared using a Mann-Whitney U test. *P < .05; **P < .01; ***P < .001; ****P < .0001. Experiments were conducted in triplicate or more. Heatmaps present the log₂ fold change (log2FC) of gene expression, using Mll-AF4⁺ pMIRH (Control) as a reference.