Mll-AF4⁺ pMIRH-130b and Mll-AF4⁺ pMIRH-128a leukemia are propagated by different leukemic stem cells. (A) Experimental design. All sorted cells were retrieved from recipients originally transplanted with total BM cells. Secondary recipients received cells retrieved from the BM of FL Mll-AF4⁺ LSK primary recipients. Tertiary recipients received cells from the BM of total BM secondary recipients. Survival curves of (B) secondary and (C) tertiary transplants of mice that received sorted HSCs/MPPs (LSK Il7r⁻ Flt3⁻), LMPPs (LSK Flt3⁺), or LK/CLPs (Lineage^-Sca1^low/-ckit⁺) from the BM of Mll-AF4⁺ pMIRH-130b sick mice. Survival curves of (D) secondary and (E) tertiary transplants of mice that received sorted HSCs/MPPs, LMPPs, LK/CLPs, or LSK Il7r⁺ from the BM of Mll-AF4⁺ pMIRH-128a sick mice. GFP chimerism in the BM of (F) secondary and (G) tertiary recipients. (H) Proportion of hematopoietic cells in secondary recipients of LMPPs from Mll-AF4⁺ pMIRH-130b sick mice. Spleen and liver weight are also indicated. (I) Spleen and (J) liver weight of sick tertiary recipients that received sorted HSCs/MPPs, LMPPs, LK/CLPs, or LSK Il7r⁺ from the BM of Mll-AF4⁺ pMIRH-130b or Mll-AF4⁺ pMIRH-128a sick mice. (K) Proportion of myeloid cells, mixed lineage cells, B-lymphoid cells, and ckit⁺ Il7r⁺ cells. (L) T-lymphoid cells in the BM of tertiary recipients of sorted cells from Mll-AF4⁺ pMIRH-130b or Mll-AF4⁺ pMIRH-128a sick mice. Proportion of (M) ckit⁺ cells and (N) HSCs/MPPs, LMPPs, and LSK Il7r⁺ cells in the BM LSK compartment of tertiary recipients of sorted cells from Mll-AF4⁺ pMIRH-130b sick mice. Graphs are presented as mean ± standard error of the mean (1 dot = 1 mouse).