AVEXIS screen identified a potential novel interaction between CD300a and CD148 receptors. (A) Normalized monobiotinylated bait proteins were arrayed, incubated with pentameric preys, washed, and detected with β-lactamase substrate (nitrocefin) using AVEXIS. (B) List of novel interactions identified in the current study. Complete information is shown in supplemental Table 3. (C) Prey s5-CD300a was screened against 250 monomeric bait proteins from our human platelet-leukocyte extracellular protein library; it detected CD148 as a positive hit. Two other promiscuous baits proteins also appeared in this screen (green text). The red dashed line represents the Z-score cutoff of 4. (D) Western blots showing expression of CD148 and CD300a bait proteins with expected molecular weights. (E) Prey CD300 family receptors and a negative control protein were screened against CD148 bait; only CD300a showed a positive interaction (n ≥ 3). COMP, cartilage oligomeric matrix protein.