Expression of hematopoietic growth factors and inflammatory cytokines in non-hematopoietic BM stromal cell subpopulations after a single LPS injection. (A) Representative FACS profiles showing non-hematopoietic cell types of the BM microenvironment, either non-depleted (upper panel) or with MACS column-based CD45/Ter119 depletion (lower panel) (AEC, SEC, PαS cells, and CAR cells). (B) Graphical scheme depicting experimental outline for the induction of LPS- or poly(I:C)–induced inflammation and subsequent isolation of non-hematopoietic BM cells. (C) Gene expression of 12 hematopoietic growth factors and inflammatory cytokines from PBS-, LPS-, or poly(I:C)–injected WT mice from 3 independent experiments. Relative expression normalized to Gapdh from qRT-PCR is depicted as a heat map. (D) Cxcl12 expression levels in BM AECs, SECs, PαS cells, and CAR cells were assessed by qRT-PCR and normalized to Gapdh. Il6 expression normalized to Gapdh in BM non-hematopoietic (CD45^–Ter119^–) GFP^pos and GFP^neg cells from Cxcl12-GFP (E) and LeprCre;GFP mice (F), respectively. *P < .05, ****P < .0001). Csf2, colony-stimulating factor 2 (granulocyte-macrophage); Csf3, colony-stimulating factor 3 (granulocyte); Flt3l, FMS-like tyrosine kinase 3 ligand; Ifna, interferon-α; Ifnb, interferon-β; Il1a, interleukin-1α; Il1b, interleukin-1β; Il3, interleukin-3; Il6, interleukin-6; i.p., intraperitoneal; Kitl, kit ligand; ns, not significant; Thpo, thrombopoietin; Tnf, tumor necrosis factor.