BsAbs induce secretion of effector molecules and expression of activation markers in CD4⁺ and CD8⁺ T cells. Lymph node–derived lymphocytes were incubated without (w/o) or with anti-CD3 antibody (CD3-Ab), one or two concentrations of CD19-BsAb (n = 32 biologically independent samples), or one concentration of CD20-BsAb (n = 14 biologically independent samples). Supernatants (A) or cells (B-C) were harvested after 7 days and analyzed by using flow cytometry or a bead-based immunoassay, respectively. In addition, the same samples were analyzed by flow cytometry at baseline. Shown in panel A are the levels of 5 different effector molecules, as indicated. Box plots in panels B and C display the proportion of the given cell subset of the total CD4⁺ and CD8⁺ T cells. The classification into responders and nonresponders refers to Figure 2H. P values were calculated between both response groups as indicated by the brackets using the two-sided Wilcoxon’s test. P values were adjusted by using the Benjamini-Hochberg procedure. ***≙ P ≤ .001; **≙ P ≤ .01; *≙ P ≤ .05; missing asterisks indicate P > .05. ns, not significant.