HGAL expression inhibits BJAB lymphoma cell growth and metastasis and prolongs mouse survival. Mice were injected intravenously with 5 × 106 luciferase-transduced human lymphoma cells from cell line BJAB. D-luciferin was injected intraperitoneally (150 mg/kg body weight) 15 minutes before acquiring images. Bioluminescence images were taken once per week. (A) Representative bioluminescent images of mice with BJAB Mock and BJAB HGAL knockout (KO) lymphoma. (B) Tumor growth curve based on photon flux intensity (Mock group, 12 mice; HGAL KO group, 19 mice) with significant difference between the mouse cohorts with BJAB Mock and BJAB HGAL KO lymphomas (P = .0038). Mice that were not injected (background; 5 mice) served as a control for background photon flux intensity. Normalized photon flux represents tumor photon flux (luminescence intensity) at the indicated time relative to photon flux measured after cell injection on day 0 that was set to value of 100. (C) Number of individual sites with lymphoma involvement in BJAB Mock and BJAB HGAL KO mice (P = .0051). (D) Kaplan-Meier plots of mouse survival demonstrating significant difference between the BJAB Mock and BJAB HGAL KO mice (P = .0051). **P <.01.