Therapeutic effect of adoptively transferred iMφs in pulmonary infected immunodeficient mice. (A) Scheme of pulmonary infection and therapeutic transplantation of iMφs in hIL-3/GM-CSF KI immunodeficient mice. Animals were infected by intrapulmonary administration of 1 × 10⁷ CFU of S aureus Newman. Four hours postinfection, the treatment group received 4 × 10⁶ human iMφs intrapulmonary (S aureus+iMφ), whereas control mice received PBS (S aureus+PBS). (B) CFU per right lung and (C) frequency of murine monocyte/granulocyte (mGr-1⁺ cells) infiltration 24 hours postinfection (data from 2 independent experiments, individual values and mean ± SD, 3-8 animals/group). (D) Histopathology scores of the left lung tissue 24 hours postinfection (individual values and mean ± SD) and (E) representative microscopy images of hematoxylin-eosin staining of the lung tissue (original magnification ×2.5 for the top and ×40 for the bottom rows, scale bar = 500 and 20 µm, respectively). *P < .05; **P < .01; ****P < .0001; ns, not significant, as determined by 1-way ANOVA with Tukey’s multiple comparisons test.