Whole transcriptome analysis of iMφs and PBMφs pre- and postinfection with S aureus. (A) Secretion of the proinflammatory cytokine IL-6 by iMφs and PBMφs before and 1 hour after stimulation with LPS. (B) Principal component analysis (PCA) of uninfected control (green) and infected (red) iMφs and PBMφs 2 hours postinfection. (C) Hierarchal heatmap clustering of differentially expressed genes (DEG, P < .05 and >2-fold change) between uninfected and 2 hour postinfection cells, and corresponding Venn diagram of shared upregulated genes 2 hours postinfection. (D) Word Cloud of the most prominent upregulated genes in iMφs associated with pro-inflammatory gene ontologies (regulation of NF-kappaB import into nucleus-GO:0042345; response to IL-1-GO:0070555; cellular response to lipopolysaccharide-GO:0032496; I-kappaB kinase/NF-kappaB signaling-GO:0007249; I-kappaB kinase/NF-kappaB signaling-inflammatory response-GO:0006954). Genes represented in more GOs are designated with a larger font. (E) Hierarchical heatmap clustering of 195 DEGs in cells 2 and 24 hours postinfection compared with uninfected cells (P < .05, >2-fold change). (F) Heatmaps representing the expression pattern of genes linked to selected GOs, comparing 2- and 24-hour infected cells to uninfected macrophages (P < .01, >2-fold change).