Gene expression profile of iMφs and PBMφs 24 hours postinfection. (A) Quantitative reverse transcriptase polymerase chain reaction analysis of pro-inflammatory cytokines and chemokine; IL1-β, IL-6, and CXCL8 expression in noninfected macrophages (Ctrl), and S aureus infected macrophages 2 and 24 hours postinfection (2h and 24h), normalized to GAPDH (n = 2 biological replicates, mean ± SD). (B) Principal component analysis (PCA) of uninfected control (green) and infected (blue) iMφs and PBMφs 24 hours postinfection. (C) Hierarchal heatmap clustering of differentially expressed genes (DEGs, P < .05 and >2-fold change) between uninfected and 24-hour postinfection cells, and Word Cloud of upregulated genes 24 hours postinfection (all 15 genes in iMφs and top 27 genes in PBMφs). Genes represented in more GOs are designated with a larger font. Bar graph of top-ranked GOs (adjusted P values 10^-3-10^-5) related to genes upregulated in PBMφs 24 hours postinfection. (D) Number of DEGs between iMφs and PBMφ 2 and 24 hours postinfection in comparison with respective uninfected control cells.