Functional analysis of clots formed with selective cleavage of fibrinopeptides. (A) Clottability of neonatal and adult fibrinogen with mid-range concentrations of fibrinopeptide cleaving enzymes. N = 4 per group. (B) AFM was used to determine stiffness for clots formed with neonatal and adult fibrinogen with mid-range concentrations of fibrinopeptides. N = 3 to 7 per group. Representative degradation curves (C) and degradation rates (D) for clots formed with venom enzymes were determined from the time taken to reach half-maximum soluble protein. N = 5 to 6 per group. Average values ± standard deviation is reported. *P < .05, **P < .01, ***P < .001, ****P < .0001.