Diagram of RNA-seq and subtype classification of patients with B-ALL. RNA-seq was performed on a subset of patients with B-ALL enrolled in the MS2003 and MS2010 studies (including a few treated as per protocol). After quality control, RNA-seq data of 377 patients qualified for analysis. Patients were classified into 20 subtypes using 4 different methods. Some subtypes may be classified with multiple methods; only the leading method is indicated (Table 1).