B-NHL spheroids are cohesive and express extracellular matrix. (A) Pictures showing the maintenance of SUDHL4 and HLY1 spheroids after capsule dissolution by incubation in Phosphate Buffered Saline (PBS) containing 1 mM EGTA. Scale bar, 100 µm. (B) Immunostaining showing the expression of extracellular matrix in cells cultured in suspension (2D) or in spheroids (3D). Extracellular matrix components were visualized by staining with anti-pan Laminin and anti-Collagen I antibodies on 5µm thick sections of spheroids embedded in paraffin. Nuclei are depicted in blue. Scale bar, 50 µm. (C) RT-qPCR revealing the upregulation of LAMC1 and COL1A expression in SUDHL4 and HLY1 cultured in 3D compared with 2D (n = 3).