Self-organization of the stromal network. (A) Representatives images of capsules containing DOHH2 with Mg and GFP-TSC at different culture time point. After fixation, 10 to 18 spheroids for each time point were placed in an Universlide. Images shown are Z-projections. TSC are shown in green and all the analyses were performed on green signal exclusively. Nuclei of DOHH2 and TSC cells were counterstained in blue with Hoechst 33258. The edges of the capsule are depicted by white dashed lines. Scale bar, 50 µm. (B-C) Sphericity and branchness indexes of green particles contained in spheroids at different times after encapsulation, respectively. To determine significant differences, an analysis of variance was performed (*P < .05, **P < .01, ***P < .001). (D) Correlation of sphericity and branchness. For each time point, small dots represent individual spheroids whereas big dots represent the average values of all spheroids for a given time point. Pearson correlation coefficient (R) was calculated using R software. The black line represents the regression line and the gray area represents the 95% confidence interval of the linear regression.