Phenotypic characterization of DOHH2 and HLY1 in the 3D coculture model. (A) The H3D (HLY1 in 3D) vs H2D (HLY1 in 2D), HT3D (HLY1 in 3D with TSC) vs H2D (HLY1 in 2D), and HT3D vs HT2D (HLY1 in 2D with TSC) gene signatures were analyzed by GSEA using HallMark MSigDB. (B) Enrichment of transcription factor (TF) targets in HLY1 signatures as determined using TRRUST database with EnrichR website. The 10 more significant predicted TF are represented. (C) The DT2D (DOHH2 in 2D with TSC) vs D2D (DOHH2 in 2D with TSC), DT3D (DOHH2 in 3D with TSC) vs D2D (DOHH2 in 2D), and DT3D vs DT2D gene signatures were analyzed by GSEA using HallMark MSigDB. (D) Enrichment of TF targets in DOHH2 signatures as determined using TRRUST database with EnrichR website. The 10 more significant predicted TF are represented. (E) Upregulated gene pathways identified by GSEA using in HallMark MSigDB in FL B cells vs centrocytes based on Affymetrix microarray data. The 10 more significant predicted pathways are represented.