GLI3R behaves as a tumor suppressor. (A) Time course of proliferation and viability for HMC1.2 cells transduced with lentiviral vectors expressing tdTomato-GLI3R or tdTomato-control, by the IncuCyte imaging system. Cell viability was determined by light scatter measurement by FACS. (B) Immunoblot of GLI3, Bcl2, c-Myc, and p21 in HMC1.2 cells transduced with the 2 tdTomato vectors indicated above. (C) Relative gene expression of GLI3, GLI1, AKT, and c-Myc in transduced HMC1.2 cells. Data are from 3 independent experiments. (D) Transcriptional profiling of the transduced HMC1.2 cells using RNA-seq. Differentially expressed genes involved in cell death (z score, 2.58), apoptosis (z score, 3.02), viability (z score, −2.46), and cell proliferation (z score, −2.27) are highlighted by the heat maps (see “Methods”). Selection of 23, 13, and 8 differentially expressed genes, respectively involved in cell proliferation, apoptosis and MC activation is represented. Green and red intensities indicate the degree of downregulation and upregulation, respectively. ****P < .0001.