Identification of a CD57⁺/CD27⁻ CD4⁺ T-cell population. (A) Clustered UMAP plot generated using 33 antibodies from the human T-cell panel (CIC-002) characterizing PBSC samples from 7 CMV donor-positive (D⁺) and 5 CMV donor-negative (D^–) transplants show 29 distinct cell clusters across all samples. Differential frequency for each cluster between D⁺ and D^– samples are shown with corresponding P (blue) and q values (red). Only 1 cluster (15) met an false discovery rate threshold of 0.2. Selected antibody results are shown to the right of the UMAP plot. (B) For each cluster, normalized antibody intensity is shown using Marker Enrichment Modeling (MEM). (C) 2 × 2 gating was performed for CD4⁺ T cells to emphasize the CD27/CD57 profile for D⁺ and D^– groups.