Effects of ELT on cell proliferation and apoptosis. The effects of ELT on cell proliferation and apoptosis were assessed on day 7 of culture using EdU incorporation and TUNEL assays, respectively. (A) Representative FACS density plots showing EdU incorporation in different cell populations. (B) Representative FACS density plots showing the TUNEL⁺ fraction in different cell populations. (C) In EdU incorporation assays, 30 µM of ELT significantly suppressed the proliferation of CD34⁺CD41⁺ and CD34⁻CD41⁺ cells cultured in iron-depleted conditions, a finding that was reversed by increased iron availability in culture. In contrast, CD34⁺/CD41⁻ cells cultured with TPO only had significantly higher EdU incorporation when cultured in iron-depleted conditions. (D) Thirty µM of ELT also induced apoptosis in all 3 cell populations, but increased iron availability did not significantly reduce the level of apoptosis. Data reflect the mean ± SD of 5 independent experiments. *P < .05; ***P < .001.