Figure 2.
ATAC is selectively required for erythroid specification from CB HSC. (A) qRT-PCR validation of SUPT20H and ZZZ3 knockdown in human CB HSC. Representative experiment for SUPT20H; mean ± SEM of 2 individual experiments for ZZZ3; gene expression relative to CTRLsh, normalized to HPRT1 housekeeping gene. Paired 2-tailed Student t test for significance *P < .05. (B) Representative sorting plot for transduced HSC and progenitor cells from CB. (C) Frequency of CFC efficiency in the HSC/multipotent progenitor (MPP), MEP, GMP, and MLP compartments transduced with ZZZ3sh. Mean ± SEM of 5 individual CB samples (4 for GMP, MLP). Two-tailed paired Student t test for significance; *P < .05. (D) Frequency of CFC efficiency in the HSC/MPP, MEP, GMP, and MLP compartments transduced with SUPT20Hsh. Mean ± SEM of 4 individual CB samples. Two-tailed paired Student t test for significance; no significant differences.

ATAC is selectively required for erythroid specification from CB HSC. (A) qRT-PCR validation of SUPT20H and ZZZ3 knockdown in human CB HSC. Representative experiment for SUPT20H; mean ± SEM of 2 individual experiments for ZZZ3; gene expression relative to CTRLsh, normalized to HPRT1 housekeeping gene. Paired 2-tailed Student t test for significance *P < .05. (B) Representative sorting plot for transduced HSC and progenitor cells from CB. (C) Frequency of CFC efficiency in the HSC/multipotent progenitor (MPP), MEP, GMP, and MLP compartments transduced with ZZZ3sh. Mean ± SEM of 5 individual CB samples (4 for GMP, MLP). Two-tailed paired Student t test for significance; *P < .05. (D) Frequency of CFC efficiency in the HSC/MPP, MEP, GMP, and MLP compartments transduced with SUPT20Hsh. Mean ± SEM of 4 individual CB samples. Two-tailed paired Student t test for significance; no significant differences.

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