American Society of Hematology

Figure 1.

Evaluation of Cast-PCR assay. (A1) Representative amplification Cast-PCR plots for wild type cfDNA samples. (A2) Representative amplification Cast-PCR plots for MYD88L265P cfDNA samples. (B1) Evaluation of Cast-PCR sensitivity by the determination of the LOD using dilutions at different ratios of MYD88L265P MWCL1 cell line and MYD88WT gDNA clinical sample. (B2) Representation of the standard curve between ΔCt and dilution factor of MYD88L265P and MYD88WT samples. (C1) Determination of Cast-PCR efficiency by using 0.5 to 1000 times serial dilutions of mutated cfDNA sample. (C2) Determination of Cast-PCR LOD based on the quantity of DNA in the reaction.

Evaluation of Cast-PCR assay. (A₁) Representative amplification Cast-PCR plots for wild type cfDNA samples. (A₂) Representative amplification Cast-PCR plots for MYD88^L265P cfDNA samples. (B₁) Evaluation of Cast-PCR sensitivity by the determination of the LOD using dilutions at different ratios of MYD88^L265P MWCL1 cell line and MYD88^WT gDNA clinical sample. (B₂) Representation of the standard curve between ΔCt and dilution factor of MYD88^L265P and MYD88^WT samples. (C₁) Determination of Cast-PCR efficiency by using 0.5 to 1000 times serial dilutions of mutated cfDNA sample. (C₂) Determination of Cast-PCR LOD based on the quantity of DNA in the reaction.

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