![IL-1 is upregulated in older BM. (A) Antibody-based protein array of whole BM lysates from 2-month-old (2mo 1-5, n = 5) and 2-year-old (2y 1-3, n = 3) WT SPF animals. Proteins that overlay with gene chip microarray analysis are indicated in bold. Heatmap represents significantly (P < .05) upregulated proteins in 2mo vs 2y BM. (B) Venn diagram illustrating the overlap of differentially regulated (2y, 2y vs 2mo, 2mo) proteins (protein array) and transcripts (gene chip microarray) in indicated WBM samples. (Protein array: 2mo, n = 5; 2y, n = 3; gene chip microarray: 2mo, n = 2; 2y, n = 2). (C) Relative expression of Il1 family genes (Il1a, Il1b, Il1r1, Il1r2, Il1ra) to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) of 2mo (n = 4-11) and 2y (n = 3-7) BM populations. LT-HSCs (Lin−Sca1+c-Kit–(LSK) CD34–Flt3–CD48–CD150+; 2mo, n = 17; 2y, n = 20), common myeloid progenitor (CMP) cells (Lin−c-Kit–(LK) CD34+FcgRneg/low; 2mo, n = 2; 2y, n = 12), granulocyte macrophage progenitor (GMP) cells (LK CD34+FcgR+; 2mo, n = 2; 2y, n = 12), mesenchymal stromal cells (MSCs) (Ter119/CD45– CD31–Sca1+CD140b+; 2mo, n = 5; 2y, n = 3), CXCL12-abundant reticular (CAR) cells (Ter119/CD45– CD31–Sca1–CD140b+; 2mo, n = 5; 2y, n = 3), endothelial cells (ECs) (Ter119/CD45– CD31+Sca1+; 2mo, n = 5; 2y, n = 3), inflammatory monocytes (iMono) (CD11b+Gr1lowLy6Chigh; 2mo, n = 11; 2y, n = 3), granulocytes (Gran) (CD11b+Gr1high; 2mo, n = 11; 2y, n = 3), macrophages [Mφ] (CD11b+F4/80+; 2mo, n = 11; 2y, n = 2), NK cells (CD11b–Gr1–CD3e–CD19–B220–NK1.1+; 2mo, n = 11; 2y, n = 3), dendritic cells (DCs) (CD11c+MHCII+; 2mo, n = 5; 2y, n = 3), B cells (CD11b–Gr1–CD3e–CD19+B220+NK1.1–; 2mo, n = 11; 2y, n = 3), plasma cells (PCs) (CD11b–Ly6C–Gr1–B220–CD3–NK1.1–CD138+; 2mo, n = 4; 2y, n = 4), and T cells (CD11b–Gr1–CD3e+CD19–B220–NK1.1–; 2mo, n = 11; 2y, n = 3). Frequencies of the cellular populations in live WBM nucleated cells from 2mo and 2y mice are indicated in percentages. Error bars represent standard error of the mean (SEM). P values were calculated by using Student t test. *P < .05; **P < .01; ***P < .001. N.D., not determined.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/139/1/10.1182_blood.2021011570/4/bloodbld2021011570f1.png?Expires=1735815829&Signature=wC5TSHsrFmMs3u2wAiHruZRTvW~-078fUdyjVJ0PwOgjoOcBXUj88WrrXucPeXthkoeAbspb3~laX55EGg4wnWgLO6lS5J15aC82pBD0t3f44bVateLDk4wz--BGxubQTnav0jldHtRFp9TjdRDFDMFbea076PwUGDcnIHbw3N7hI2ewu~p3Cz5Ix2GP4JutcM96IDZnrqtk8dUX-Kbg8u1RXmqX4Mj6L1FTozQ2lWkiIM9KMvFsOLFiLw403-AZyxun4JpqIOOssqHWmX1oxwf-nMDKpSgaj1jzPeJaXW7OqVa8JSD0slTjc50he59P~Z98jCE2haMdG4yofCBHLQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-1 is upregulated in older BM. (A) Antibody-based protein array of whole BM lysates from 2-month-old (2mo 1-5, n = 5) and 2-year-old (2y 1-3, n = 3) WT SPF animals. Proteins that overlay with gene chip microarray analysis are indicated in bold. Heatmap represents significantly (P < .05) upregulated proteins in 2mo vs 2y BM. (B) Venn diagram illustrating the overlap of differentially regulated (2y, 2y vs 2mo, 2mo) proteins (protein array) and transcripts (gene chip microarray) in indicated WBM samples. (Protein array: 2mo, n = 5; 2y, n = 3; gene chip microarray: 2mo, n = 2; 2y, n = 2). (C) Relative expression of Il1 family genes (Il1a, Il1b, Il1r1, Il1r2, Il1ra) to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) of 2mo (n = 4-11) and 2y (n = 3-7) BM populations. LT-HSCs (Lin−Sca1+c-Kit–(LSK) CD34–Flt3–CD48–CD150+; 2mo, n = 17; 2y, n = 20), common myeloid progenitor (CMP) cells (Lin−c-Kit–(LK) CD34+FcgRneg/low; 2mo, n = 2; 2y, n = 12), granulocyte macrophage progenitor (GMP) cells (LK CD34+FcgR+; 2mo, n = 2; 2y, n = 12), mesenchymal stromal cells (MSCs) (Ter119/CD45– CD31–Sca1+CD140b+; 2mo, n = 5; 2y, n = 3), CXCL12-abundant reticular (CAR) cells (Ter119/CD45– CD31–Sca1–CD140b+; 2mo, n = 5; 2y, n = 3), endothelial cells (ECs) (Ter119/CD45– CD31+Sca1+; 2mo, n = 5; 2y, n = 3), inflammatory monocytes (iMono) (CD11b+Gr1lowLy6Chigh; 2mo, n = 11; 2y, n = 3), granulocytes (Gran) (CD11b+Gr1high; 2mo, n = 11; 2y, n = 3), macrophages [Mφ] (CD11b+F4/80+; 2mo, n = 11; 2y, n = 2), NK cells (CD11b–Gr1–CD3e–CD19–B220–NK1.1+; 2mo, n = 11; 2y, n = 3), dendritic cells (DCs) (CD11c+MHCII+; 2mo, n = 5; 2y, n = 3), B cells (CD11b–Gr1–CD3e–CD19+B220+NK1.1–; 2mo, n = 11; 2y, n = 3), plasma cells (PCs) (CD11b–Ly6C–Gr1–B220–CD3–NK1.1–CD138+; 2mo, n = 4; 2y, n = 4), and T cells (CD11b–Gr1–CD3e+CD19–B220–NK1.1–; 2mo, n = 11; 2y, n = 3). Frequencies of the cellular populations in live WBM nucleated cells from 2mo and 2y mice are indicated in percentages. Error bars represent standard error of the mean (SEM). P values were calculated by using Student t test. *P < .05; **P < .01; ***P < .001. N.D., not determined.