Figure 3.
IL-1 is decreased in older WT GF mice, and WT GF and IL-1R1KO SPF mice show a reduced aging-associated increase of neutrophils, platelets, and phenotypic HSCs. (A) Concentration of IL-1a and IL-1b in BM and PB lysates in WT SPF, IL-1R1KO SPF, and WT GF animals. For 2mo mice, BM lysate: IL-1a, n = 32 WT, 8 IL-1R1KO, 17 GF; IL-1b, n = 16 WT, 8 IL-1R1KO, 20 GF; PB lysate: IL-1a, n = 34 WT, 9 IL-1R1KO, 4 GF; IL-1b, n = 26 WT, 9 IL-1R1KO, 4 GF. For 1y mice, BM lysate: IL-1a, n = 15 WT, 8 IL-1R1KO, 10 GF; IL-1b, n = 15 WT, 8 IL-1R1KO, 10 GF; PB lysate: IL-1a, n = 12 WT, 6 IL-1R1KO, 6 GF; IL-1b, n = 19 WT, 6 IL-1R1KO, 6 GF. For 2y mice, BM lysate: IL-1a, n = 35 WT, 10 IL-1R1KO; IL-1b, n = 22 WT, 11 IL-1R1KO; PB lysate: IL-1a, n = 30 WT, 10 IL-1R1KO; IL-1b, n = 29 WT, 10 IL-1R1KO. (B) PB cell counts in WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo mice, n = 26 WT, 26 IL-1R1KO, 12 GF; 1y mice, n = 36 WT, 19 IL-1R1KO, 13 GF; and for 2y mice, n = 30 WT, 22 IL-1R1KO. (C) Frequency of phenotypic LT-HSCs (LKS CD34–Flt3–CD48–CD150+), MPP1 (LKS CD34+Flt3–CD48–CD150+), and LT-HSC EPCR+CD41+ subpopulation (LKS CD34–Flt3–CD48–CD150+EPCR+CD41+) in BM of WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo mice, LT-HSCs: n = 12 WT, 12 IL-1R1KO, 8 GF; MPP1: n = 9 WT, 12 IL-1R1KO, 8 GF; LT-HSC EPCR+CD41+: n = 9 WT, 12 IL-1R1KO, 8 GF. For 1y mice, LT-HSCs: n = 17 WT, 9 IL-1R1KO, 7 GF; MPP1: n = 13 WT, 9 IL-1R1KO, 8 GF; LT-HSC EPCR+CD41+: n = 13 WT, 9 IL-1R1KO, 8 GF. For 2y mice, LT-HSCs: n = 12 WT, 10 IL-1R1KO; MPP1: n = 8 WT, 10 IL-1R1KO; LT-HSC EPCR+CD41+: n = 8 WT, 10 IL-1R1KO. (D) Cell cycle profiling of phenotypic LT-HSCs (LKS CD34–Flt3–CD48–CD150+) from WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo, n = 5 WT, 4 IL-1R1KO, 4 GF. For 1y mice, n = 5 WT, 4 IL-1R1KO, 6 GF. For 2y mice, n = 5 WT, 7 IL-1R1KO. Error bars represent standard deviation (SD) in all panels. P values were calculated using Student t test. *P < .05; **P < .01; ***P < .001; ****P < .0001. HGB, hemoglobin; N.A., not applicable; PLT, platelet; RBC, red blood cell; WBC, white blood cell.

IL-1 is decreased in older WT GF mice, and WT GF and IL-1R1KO SPF mice show a reduced aging-associated increase of neutrophils, platelets, and phenotypic HSCs. (A) Concentration of IL-1a and IL-1b in BM and PB lysates in WT SPF, IL-1R1KO SPF, and WT GF animals. For 2mo mice, BM lysate: IL-1a, n = 32 WT, 8 IL-1R1KO, 17 GF; IL-1b, n = 16 WT, 8 IL-1R1KO, 20 GF; PB lysate: IL-1a, n = 34 WT, 9 IL-1R1KO, 4 GF; IL-1b, n = 26 WT, 9 IL-1R1KO, 4 GF. For 1y mice, BM lysate: IL-1a, n = 15 WT, 8 IL-1R1KO, 10 GF; IL-1b, n = 15 WT, 8 IL-1R1KO, 10 GF; PB lysate: IL-1a, n = 12 WT, 6 IL-1R1KO, 6 GF; IL-1b, n = 19 WT, 6 IL-1R1KO, 6 GF. For 2y mice, BM lysate: IL-1a, n = 35 WT, 10 IL-1R1KO; IL-1b, n = 22 WT, 11 IL-1R1KO; PB lysate: IL-1a, n = 30 WT, 10 IL-1R1KO; IL-1b, n = 29 WT, 10 IL-1R1KO. (B) PB cell counts in WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo mice, n = 26 WT, 26 IL-1R1KO, 12 GF; 1y mice, n = 36 WT, 19 IL-1R1KO, 13 GF; and for 2y mice, n = 30 WT, 22 IL-1R1KO. (C) Frequency of phenotypic LT-HSCs (LKS CD34–Flt3–CD48–CD150+), MPP1 (LKS CD34+Flt3–CD48–CD150+), and LT-HSC EPCR+CD41+ subpopulation (LKS CD34–Flt3–CD48–CD150+EPCR+CD41+) in BM of WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo mice, LT-HSCs: n = 12 WT, 12 IL-1R1KO, 8 GF; MPP1: n = 9 WT, 12 IL-1R1KO, 8 GF; LT-HSC EPCR+CD41+: n = 9 WT, 12 IL-1R1KO, 8 GF. For 1y mice, LT-HSCs: n = 17 WT, 9 IL-1R1KO, 7 GF; MPP1: n = 13 WT, 9 IL-1R1KO, 8 GF; LT-HSC EPCR+CD41+: n = 13 WT, 9 IL-1R1KO, 8 GF. For 2y mice, LT-HSCs: n = 12 WT, 10 IL-1R1KO; MPP1: n = 8 WT, 10 IL-1R1KO; LT-HSC EPCR+CD41+: n = 8 WT, 10 IL-1R1KO. (D) Cell cycle profiling of phenotypic LT-HSCs (LKS CD34–Flt3–CD48–CD150+) from WT SPF, IL-1R1KO SPF, and WT GF mice. For 2mo, n = 5 WT, 4 IL-1R1KO, 4 GF. For 1y mice, n = 5 WT, 4 IL-1R1KO, 6 GF. For 2y mice, n = 5 WT, 7 IL-1R1KO. Error bars represent standard deviation (SD) in all panels. P values were calculated using Student t test. *P < .05; **P < .01; ***P < .001; ****P < .0001. HGB, hemoglobin; N.A., not applicable; PLT, platelet; RBC, red blood cell; WBC, white blood cell.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal