Association of ASNS methylation with l-asparaginase sensitivity in T-ALL cell lines. (A) Dose–response curves of l-asparaginase sensitivity in 22 T-ALL cell lines. The colors of the curves show percent methylation of ASNS in each cell line. (B) Dose–response curves of l-asparaginase sensitivity in 3 groups of T-ALL cell lines with different ASNS methylation status. Box plots indicate cell viabilities in ASNS highly (red), intermediately (yellow), and weakly (blue) methylated cell lines at each concentration. (C) Association of ASNS methylation with asparaginase sensitivity in T-ALL cell lines. In the left panel, the horizontal axis indicates the percent methylation of the ASNS gene and the vertical axis indicates the log IC₅₀ value of asparaginase. The correlation coefficient and P value in the Spearman correlation test are shown. In the right panel, the log IC₅₀ value of asparaginase is compared among ASNS highly methylated (red), intermediately methylated (yellow), and weakly methylated (blue) cell lines. Asterisks indicate significance in the Mann-Whitney U test. ***P < .001. N.S., not significant.