Recognition of tumor specific antigens over time. Recovery of antigen-specific T cells and antigen spreading as detected by IFNγ ELISPOT assay in patients receiving TAA-T and nivolumab in responders (A-D) and nonresponders (E-F). For each figure, reactivity to the targeted antigens WT1, PRAME, and Survivin is shown in panel i and nontargeted antigens MAGE family, SSX2, and SOX2 in panel ii.