Complete remission from active AML after mLST infusion. MRI images of AML invading bone before (A) and after (B) mLST infusion. (C) Relapsed AML cells (CD33⁺ blasts; IHC). Hematoxylin and eosin; original magnification ×400. Cells were surrounded by a dense infiltrate of CD3⁺ T cells. IHC; original magnification ×10. Changes in frequency of circulating mLSTs after infusion, as measured by IFN-γ ELISpot (D) and polyclonality of circulating WT1-specific T cells, as estimated by ICS (E) performed on fresh PBMCs at the 6-month time point. (F) Change in the frequency of mLST-derived TCR clones represented as fold change from baseline in the repertoire frequency.