Disruption of B lymphopoiesis and double-stranded DNA breaks in B cells are correlated with myeloid expansion in Il10^−/− mice. Analysis of immune cell lineages and cytokines in 8- to 12-week-old Il10^+/+ and Il10^−/− mice. (A) Representative flow cytometry plots show the percentage of CD11b⁺ CD19⁻ myeloid cells and CD19⁺ B cells of FVB/N and Il10^−/− bone marrow (BM) gated on single live cells (left panel). Bar graph summarizes the number of CD19⁺ B, CD3⁺ T, and CD11b⁺ CD19⁻ myeloid cells in the BM (right panel). (B) Number of pro-B cells (B220⁺CD19⁺IgM⁻IgD⁻CD43⁺), pre-B cells (B220⁺CD19⁺IgM⁻IgD⁻CD43⁻), IgM⁺ immature B cells (B220⁺CD19⁺IgM⁺IgD⁻), and MR B cells (B220⁺CD19⁺IgM⁺IgD⁺) in the BM. (C) Percentage of B-cell subsets in B220⁺CD19⁺ BM. (D) Number of neutrophils (CD11b⁺CD19⁻ Gr1^hiLy6C^lo) and monocytes (CD11b⁺CD19⁻Gr1^loLy6C^hi) in the BM. (E) Percentage of myeloid subsets in single live BM cells. Percentage of γH2AX⁺ cells among total BM B cells (F) and BM B-cell subsets (G). (H) Absolute plasma concentrations of a selected panel of myeloid, proinflammatory, and T-cell regulatory cytokines detected by a bead-based multiplex Luminex assay. Plasmas of all mice were assayed simultaneously. Nominal P values are presented for cytokine data. Data in (A-G) are representative of 3 experiments. Error bars represent mean ± standard deviation. *P ≤ .05, **P ≤ .01, ***P ≤ .001, ****P ≤ .001. Statistical tests used are described in Methods.