The perturbed homeostasis observed in the NK^imm group is confined to NK cells. Systems level immune mapping by flow and mass cytometry. (A) Differences in the density of cells from each group visualized in a tSNE map. (B) Relative expression of selected markers for main immune cell lineages and (C) other assessed markers shown on a t-SNE clustering of sampled events from all individuals (n = 29). Aggregated group level analysis of major immune cell subsets (D) and subset-level (E-G) analyses. Data shown in heat maps are depicted as median log₂-fold change as compared with HD. Frequencies were drawn from the parent population. HD (n = 10), NP (n = 10), and NK^imm (n = 9). *P < .05; **P < .01, by nonparametric test. t-SNE, t-distributed stochastic neighbor embedding.