Induction of ATC by trauma and shock. Mice were subjected to a midline laparotomy denoted as trauma (T), acute blood withdrawal (approximately 500 µL) to induce shock (S), or both trauma and shock (TS). Control baseline (BL) mice were catheterized but were not subjected to trauma or shock (supplemental Figure 1). (A) MAP, monitored with a pressure probe inserted into the carotid artery (n = 3). (B) APTT at 60 minutes (n = 5-7). (C) APC plasma levels measured at 60 minutes (n = 5-8). Results are shown as mean ± SD. Statistical significance was determined by (B) Kruskal-Wallis 1-way ANOVA with Dunn’s multiple comparisons test and (C) 1-way ANOVA with Dunnett’s multiple comparisons test. ****P < .0001.