Development of ATC after severe bleeding and prevention by ^superFVa prophylaxis. Severe bleeding was induced by a midline laparotomy followed by liver laceration (LL) involving the removal of 75% of the left lobe of the liver (supplemental Figure 4). Control mice (No LL) underwent the same procedures except liver laceration. In some experiments, a pressure probe was inserted into the carotid artery to gain information about the MAP. Mice received ^superFVa (SFVa) prophylaxis (0.4 or 0.8 mg/kg) or vehicle control (0 mg/kg SFVa) as a bolus IV administration (100 µL) shortly before liver laceration. Plasma samples were collected 60 minutes after liver laceration. (A) Weight of the excised liver (n = 10). (B) Blood loss after 60 minutes (n = 5-7). (C) MAP, monitored using a pressure probe inserted into the carotid artery (n = 3). MAP after trauma and shock (TS, supplemental Figure 1) is shown for comparison. (D) APTT at 60 minutes (n = 5-7). Results are shown as mean ± SD. Statistical significance was determined by Kruskal-Wallis 1-way ANOVA with Dunn’s multiple comparisons test. **P < .01; ***P < .001; ****P < .0001; ns, not significant.