Changes in ATC-associated coagulation parameters by ^superFVa prophylaxis. Severe bleeding was induced by a midline laparotomy followed by liver laceration (LL) involving the removal of 75% of the left lobe of the liver (supplemental Figure 4). Control mice (No LL) underwent the same procedures except liver laceration. Mice received ^superFVa (SFVa) prophylaxis (0.4 or 0.8 mg/kg) or vehicle control (0 mg/kg SFVa) as a bolus IV administration (100 µL) shortly before liver laceration. Plasma samples were collected 60 minutes after liver laceration. Shown are (A) APC plasma levels (n = 5-10) and plasma activity levels of (B) FV (n = 4-7), (C) FVIII (n = 4-5), and (D) fibrinogen (n = 3-6). Results are shown as mean ± SD. Statistical significance was determined by 1-way ANOVA with Dunnett’s multiple comparisons test. ****P < .0001.