A synthetic plasmid control for the LSC17 LDT performs equivalently to patient RNA samples. (A) Box-and-whisker plots showing log₂-transformed read counts for each of the 17 score genes (filled boxes) and the 12 housekeeping genes (white boxes) on 9 consecutive runs of the LSC17 LDT measured in a patient sample with high LSC17 score (red boxes), a patient sample with low LSC17 score (blue boxes), and the synthetic plasmid control (green boxes). Each box represents the replicate read counts from a single probe. (B) Plot showing the LSC17 scores calculated from the data shown in panel A (high LSC17 score, red; low LSC17 score, blue; synthetic control, green.