Differentially expressed genes in IP-ECFCs and the enriched GO-biological process terms. (A) Volcano plot illustrates significantly DEGs in IP-ECFCs in which –log10 (p) is plotted against the mean differences. Green dots represent the upregulated genes and red dots represent downregulated genes. Numbers of genes upregulated or downregulated are denoted. Dashed lines mark the threshold of statistical significance. DEGs were considered significant when P < .05 and fold-change was > 2 in respect of healthy control ECFC samples/or absolute log2FC (mean difference) was > 1. A negative mean difference value indicates lower expression in healthy controls and a positive mean difference indicates lower expression in IP-ECFCs. The blue dots illustrate the expression of WPB cargos, which are either upregulated (CXCL8, IL6, CXCL1, and SELP) or are expressed normally (VWF, Ang2, CCL2, CD63, and IGFBP7). (B) Graphs show the expression level (represented by TMM) of WPB constituents in healthy as well as index patient ECFCs. The mean difference values (Diff), calculating a difference between the expression of healthy controls and the IP, as well as P values are specified above the columns. There was no significant difference in expression (Diff) of the VWF, Ang2, CCL2, CD63, and IGFBP7 between healthy and IP-ECFCs (P > .05), though the expression of IL-8, CXCL1, IL-6, and P-selectin in IP was upregulated, demonstrating significant Diff (P < .05). (C) The table displays the list of top 10 enriched GO-biological process terms as well as the examples of the representative genes (green: upregulated genes; red: downregulated genes), and their Fold Enrichment Score estimated after carrying out GO enrichment analysis using the GO Consortium tool. TMM, trimmed mean of the M-values.