Gata2 +9.5^+/− BM cells display decreased reconstitution in primary recipients. A total of 1 × 10⁶ CD45.2⁺ BM cells from 6-week-old WT C57BL/6J (Control), Gata2 +9.5^+/− (+9.5^+/−), and Gata2^+/− (Gata2^+/−) mice were mixed with 1 × 10⁶ CD45.1⁺ competitor BM cells and injected into lethally irradiated recipients (CD45.1⁺). (A) Quantification of donor-derived myeloid, T, and B cells in recipient peripheral blood every 4 weeks after transplantation. (B-D) Terminal evaluation was performed in primary recipients 16 weeks after transplant. (B) Quantification of donor-derived cells in BM and SP of primary recipients. (C) Quantification of donor-derived myeloid, T, and B cells in BM and SP of primary recipients. (D) Quantification of donor-derived HSCs and MPPs in BM of primary recipients. The results are presented as means ± SD. *P < .05; **P < .01; ***P < .001. (E-G) Recipients were killed at 8 weeks after transplant. (E) Quantification of donor-derived MPP1, MPP2, and MPP3; CLPs; and CCR9⁺ CLPs in BM. (F) Quantification of thymic cell numbers. (G) Quantification of donor-derived T cells in thymus. The results are presented as means + SD. *P < .05; **P < .01; ***P < .001.