ESTs generated targeting latency type III vs latency type II EBV-associated lymphoproliferative disorders. Mononuclear cells are isolated from peripheral blood from autologous or allogeneic donor. (A) Laboratory-strain EBV infects peripheral blood mononuclear cells (PBMCs) to generate LCLs. LCLs express all EBV-encoded proteins and are used as antigen-presenting cells in coculture with PBMCs to expand ESTs, which target immunodominant EBNA3 and other EBV latent antigens. (B) Laboratory-strain EBV infects PBMCs to generate LCLs, which are transduced with adenoviral vector expressing LMP1 and LMP2. Monocytes are also transduced with adenoviral vector expressing LMP1 and LMP2 and cocultured with isolated T cells. Transduced LCLs are used in second stimulation to further expand LMP-specific T cells.