HL in individuals infected by HIV. Hodgkin and Reed-Sternberg cells (HRS) in HL of individual infected by HIV express CD15, CD30, CD40, and MUM1/IRF4 and LMP1 (EBV-type II latency) (A) Several HRS cells are seen (in the red circle) within a mixed inflammatory microenvironment. These cells express CD30 (inset). (B) EBV-infected tumor cells are demonstrated by EBER in situ hybridization and LMP1 immunostaining. CD30 and LMP1, immunohistochemistry, hematoxylin counterstain; EBER, in situ hybridization, nuclear fast red counterstaining. Original magnification, ×400. Images were taken using a Nikon Eclipse 80i microscope with a Plan Fluor 40×/0.75 objective and Nikon digital sight DS-Fi1 camera equipped with control unit-DS-L2. Images were processed using Adobe Photoshop 6.