Resolution of the tumor microenvironment by gene expression analysis. (A) Heatmap of scaled activity score values related to microenvironment signatures. HCPC clustering reveals 2 major microenvironment classes (“immune-suppressive” and “immune-silent”) in the SMZL cohort. The differential expression of the microenvironment signatures is also represented as density plots on the right. (B) Alluvial plot showing sample distribution among microenvironment classes and mutational clusters. (C) The heatmap shows the percentages of patients mutated for the top mutated genes in the 2 microenvironment classes. Size and color intensities of the circles are proportional to the prevalence of each variable in the microenvironment class. The asterisk shows statistical significant differences (Fisher’s exact test P < .05).