Orthogonal validations of the microenvironment composition. (A) Digital cytometry experiments. Gene expression data were used to infer the cell composition (covering 22 hematopoietic cellular populations) of spleen tissues. Staked barplots represent the individual cell compositions (scaled to a total of 100%). Samples (on the x-axis) are ordered according to the 2 major microenvironment classes. (B) The heatmap shows the distribution of selected TMA markers across the 2 microenvironment classes. A red color indicates the positivity of the marker, a white color indicates the negativity of the marker, and gray color indicates missing data. The barplot on the left shows the -log₁₀P value of a 1-sided Fisher’s exact test between the 2 classes for each marker. (C) CD3 (T cell), CD163 (M2 macrophages), CD21 (FDC), PD1, PDL1 expression in TMA cores from SMZL spleen section. (D) The heatmap shows pairwise Pearson correlation coefficients between TMA markers. Red cells indicate negative correlation, and blue cells indicate positive correlation.